Overexpression analysis of EMV2 gene coding for late embryogenesis abundant protein from Vigna radiata (Wilczek)
Автор: Rajesh S., Raveendran M., Sivakumar P., Manickam A.
Журнал: Журнал стресс-физиологии и биохимии @jspb
Статья в выпуске: 3-4 т.4, 2008 года.
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Late embryogenesis abundant (LEA) proteins are speculated to protect against water stress deficit in plants. An over expression system for mungbean late embryogenesis abundant protein, emv2 was constructed in a pET29a vector, designated pET-emv2 which is responsible for higher expression under the transcriptional/translational control of T7/lac promoter incorporated in the Escherichia coli BL21 (DE3).Induction protocol was optimized for pET recombinants harboring the target gene. Overexpressed EMV2 protein was purified to homogeneity and the protein profile monitored by SDS-PAGE.
Lea protein, overexpression, vigna, desiccation
Короткий адрес: https://sciup.org/14323468
IDR: 14323468
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