Curcumin as an anti-proliferative agent in breast cancer through RASSF1A, BAX, and CASPASE-3 protein

Curcumin as an anti-proliferative agent in breast cancer through RASSF1A, BAX, and CASPASE-3 protein

Автор: Rahmah N.A., Harliansyah H., Suyatna F.D., Kanoko M., Rustamadji P., Prihartono J., Bustami A., Haryono S.J., Hernowo B.S.

Журнал: Сибирский онкологический журнал @siboncoj

Рубрика: Лабораторные и экспериментальные исследования

Статья в выпуске: 6 т.21, 2022 года.

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Background. Curcumin is a polyphenol that has pharmacological activity that can inhibit tumor cell growth and induce apoptosis through various mechanisms. However, the specific mechanism of curcumin cytotoxicity remains controversial because of many anti- and pro-apoptotic signaling pathways in various cell types. This study aims to examine the relationship among curcumin on RASSF1A, Bax protein levels, and caspase-3 activity in supporting the apoptotic mechanism in CSA03 breast cancer cells. Material and Methods. Curcumin administration to cancer cells is based on differences in dosage with 24-hour incubation. Cytotoxicity after curcumin administration was determined using MTS. RASSF1A and Bax protein levels were tested through ELiSA. Caspase-3 activity was used to determine apoptosis and was tested using flow cytometry. Results. The results indicated that curcumin had a cytotoxicity effect of 40.85 |jg/mL. At concentrations of 40 |jg/mL and 50 jg/mL, curcumin increases levels of protein RASSF1A (A = 26.53% and 47.35%, respectively), Bax (A = 48.79% and 386.15%), and caspase-3 (A = 1,678.51% and 1,871.889%) significantly. Conclusions. Curcumin exhibits anti-proliferative activity and apoptotic (Caspase-3) effects through activation of RASSF1A and Bax.

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Bax, caspase-3, curcumin, csa03, rassf1a

Короткий адрес: https://sciup.org/140296698

IDR: 140296698   |   DOI: 10.21294/1814-4861-2022-21-6-91-98

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