Design of highly specific oligonucleotide primers and probes for the identification of non-tuberculosis mycobacteria

The purpose of this work was to develop highly specific oligonucleotide primers and probes for the identification of non-tuberculosis mycobacteria in pathological material taken from tuberculin-responsive cattle. The article presents the result of the development and design of a set of oligonucleotide primers and probes, research of their specificity and sensitivity. The primers and probes developed by us for the identification of non-tuberculosis mycobacteria in order to differentiate nonspecific reactions to tuberculin in cattle have high specificity and sensitivity.