Evaluation of biocompatibility of bacterial isolates-components of bioconservants

The aim of this work was to study the relationship between the isolates of Bacillus subtilis EFS, Lactobacillus plantarum, Propionibacterium freudenreichii, Lactococcus lactis, and Streptococcus lactis, which have the potential for the development of a bacterial preparation that improves the safety of plant-based feed during harvesting and storage. The possibility of using the tested isolates as part of the association was identified by the drip method, which is high quality and most suitable for primary testing. The method involved direct joint cultivation of microorganisms on the surface of a dense nutrient medium and visual assessment of signs of suppression or enhancement of the growth of one culture by another. As a result of the study, the following types of interactions between microorganisms were identified: neutrality, contact progression, antagonism, and contact regression. For inclusion in the association, isolates that showed neutrality and contact progression to each other were selected. The corresponding types of relationships were characteristic of B. subtilis EFS, L. plantarum, P. freudenreichii, and L. lactis isolates. Based on this, six recipes were formed that are promising for further research in order to develop a biological preparation that helps to improve the safety of plant-based feed during harvesting and storage.