Microsatellite loci for identification of oil flax varieties of the breeding of V.S. Pustovoit All-Russian Research Institute of Oil Crops: selection of informative primers and optimal conditions for DNA PCR

The aim of the research was the selection of informative microsatellite loci and optimal conditions for DNA PCR in order to create molecular genetic passports of oil flax varieties of the breeding of V.S. Pustovoit All-Russian Research Institute of Oil Crops. Eight entries of oil flax of the laboratory of oil flax breeding of V.S. Pustovoit All-Russian Research Institute of Oil Crops became the material for the research: VNIIMK 620, К4193, К4194, К4195, К4196, К4197, К4198, К4199. The DNA was isolated from the plants at the stage of appearance of 4-6 leaves using CTAB buffer. We selected ten microsatellite loci for the research. As a result, the optimal annealing temperature was selected for each pair of primers in accordance with their nucleotide sequence. It varied from 58 to 60 °C. DNA PCR with these primers revealed 11 microsatellite loci in eight flax genotypes. Depending on the locus, the number of alleles varied from 2 to 4 (on average, 3 alleles per locus). The efficient number of ne alleles was from 1...