Effects of administration conjugate ß-endorphin with histone H2a on behavioral and vegetative indices in rats

The effects of administration conjugate ß-endorphin (ß-E) with histone H2a on behavioral and vegetative indices in rats were evaluated. The high biological activity of the synthesized ß-E conjugate with histone H2a is shown. It was found that changes in blood pressure, body temperature and total motor activity in intravenous and intracerebral injections of ß-E conjugate with histone H2a are analogous to ß-E effects introduced into lateral ventricles of the brain. The similarity in action of peripheral and intracerebral injections of ß-E conjugate with histone H2a is revealed, which is expressed in long-term phase changes in pain sensitivity, food intake. It was found that the distribution of conjugate of the labeled 125I ß-E with histone H2a in organs and tissues in rats differs significantly from the distribution of native 125I-ß-E. The content of labeled conjugate in brain is 3-4 times higher than content of native ß-E, which indicates its increased permeability through the blood-brain barrier.