Study of the effects of lithium salts in the presence of ethanol on the blood plasma product of oxidative DNA damage of healthy persons and alcoholic patients

The effects of lithium ascorbate and lithium succinate on the level of the marker of oxidative DNA damage - 8-hydroxy-2'-deoxyguanosine (8-OH-dG) - were studied in blood plasma of healthy persons and alcoholic patients in the presence of 0.5% ethanol in vitro. Measurement of 8-OH-dG was performed by enzyme immunoassay. Elevated concentration of 8-OH-dG in blood plasma of alcoholic patients in comparison with healthy persons was found. Ethanol in used concentration, as well as lithium salts against its background did not exert considerable effect on the level of blood plasma 8-OH-dG both in healthy persons and in alcoholic patients. It was concluded that differently from blood plasma proteins and lipids DNA was resistant to damaging impact of ethanol in the experimental conditions used. Lithium salts also did not exert any effect on 8-OH-dG of blood plasma.