Informational characteristics of microbial biofilms formed by clinical strains of Klebsiella pneumoniae in vitro on the surface of the cover glass

Purpose Obtaining quantitative and informational characteristics of biofilms formed by clinical strains of Klebsiella pneumoniae in vitro on the surface of a cover glass. Materials and methods In vitro biofilm formation on the surface of the cover glass was studied for clinical Klebsiella pneumoniae strains, isolated in a monoculture (ESBL +) (n = 3) and in associations with Staphylococcus aureus (n = 6) in 9 patients with chronic osteomyelitis of long bones harvrested from fistulae in the preoperative period or from the infection focus during surgery. Results Monocultures of K. pneumoniae (BLRS +) differed by their lower adhesive ability when compared to strains of K. pneumoniae isolated from associations with S. aureus. The highest adhesive activity on the surface of the cover glass was observed in a mixed culture of K. pneumoniae + S. aureus. Informational characteristics depended on the type of biofilms formed. Common to biofilms was the absence of changes in the maximum possible structural diversity. Significant differences between the existing structural diversity of biofilms formed by monocultures of K. pneumonia, K. pneumonia isolated from associations and a mixed culture of K. pneumoniae + S. aureus were noted. Conclusion The absence of pronounced variability of information indicators during the experiment within each microbial community indicates the tendecy of all systems of emerging biofilms to preserve stability.