Rapid development of homozygous lines through culture of isolated microspores in leafy crops of Brassicaceae burnett

Автор: Domblides Elena A., Chichvarina Olga A., Minejkina Anna I., Kurbakov Evgeniy L., Kharchenko Viktor A., Domblides Arthur S., Soldatenko Alexey V.

Журнал: Овощи России @vegetables

Рубрика: Селекция и семеноводство сельскохозяйственных растений

Статья в выпуске: 4 (48), 2019 года.

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Relevance Biotechnological methods are generally used to speed up breeding programs and to enhance genetic diversity, so the culture of isolated microspore in vitro can be regarded as one of very suitable methods. Nontraditional and uncommon vegetable crops belonging to Brassicaceae Burnett. are becoming more popular. Methods Accessions of sarepta mustard (Brassica juncea L. Czern.) and rocket salad (Eruca sativa Mill.) were taken for the study with the aim to optimize the basic protocol for these species. Results As a result of the study the optimum cultivation conditions have been determined for the species. Sizes of buds 2.5-3.5 mm long for sarepta mustard and 7.0-7.5 long for rocket salad which were used for cultivation had been experimentally defined. It was also shown that the cold pretreatment had improved the embryo yield. The nutritional NLN-13 medium with pH 6.1 and pretreatment at 32°C during a cultivation day had been shown to be more favorable for all accessions...


Культура микроспор in vitro, sarepta mustard, rocket salad, culture of isolated microspores in vitro, homozygous lines

Короткий адрес: https://readera.org/140240750

IDR: 140240750   |   DOI: 10.18619/2072-9146-2019-4-8-12

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