Improving technology of clonal micropropagation of arctic bramble (Rubus arcticus L.)

The article presents the results of studies on the type of sterilizers that influenced the survival of explants of the arctic bramble (Rubus arcticus L.) in culture in vitro. The studies are conducted in 2016-2018 in the Central European Forest Experiment Station VNIILM and Kostroma State Agricultural Academy. The influence of the composition of the nutrient medium and the addition of growth-regulating substances to it at the stages of «micro-reproduction itself» and «rooting of propagated shoots in vitro», as well as the composition of the substrate at the ex vitro adaptation stage during clonal micro-reproduction of the arctic bramble. The effect of auxins of IMC and Ecogel is studied when an arctic bramble added to the nutrient medium on organogenesis. The most active rooting took place in the variant with the addition of an IMC. Cloning of the arctic bramble is advisable to carry out on a nutrient medium 1/2 MS. The effect of various sterilizers on the survival of explants, growth regulators - on changes in the biometric indicators of the arctic bramble in vitro and the type of substrate - on the survival of ex vitro are studied. Recommendations for optimization of cloning of an arctic bramble at different stages.