A complex of biotechnological techniques for breeding program in carrot (daucus carota L.)

The biotechnological techniques that have been elaborated for carrot breeding include the following elements: the methods of initial explant sterilization; regeneration under controlled condition for callus tissue and zygotic embryoids; anther and unpollinated ovule cultivation in vitro; genetic transformation. Mentioned biotechnological approaches were applied to develop the cultivar of garden carrot Sonata, promising hybrids F1 and family population of transgenic plants carrying the high resistance to Fusarium avenaceum.