Production of doubled haploids in cucumber

Автор: Domblides Elena A., Belov Sergey N., Soldatenko Alexey V., Pivovarov Victor F.

Журнал: Овощи России @vegetables

Рубрика: Селекция и семеноводство сельскохозяйственных растений

Статья в выпуске: 5 (49), 2019 года.

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Implementation of cell technologies has essentially improved the plant breeding process in agricultural crops in the world. The production of pure lines in cultivated crops, particularly among cross-pollinated species such as cucumber (Cucumis sativus L.) requires much time, labor and expense. Thus, the use of DH-plants for production of fully homozygous lines for one year becomes a very promising method for near cucumber breeding program. The major factor limiting the wide use of DH is a lack of effective protocol for large-scale plant production. In this review the historical facts with description of three main methods of DH-plant production were presented. By now these three methods have been such as parthenogenesis in situ induced by pollination with irradiated or chemically treated pollen; androgenesis in vitro including anther and isolated microspore cultivation in vitro; gynogenesis through ovule cultivation in vitro. Comparative analysis of published data with regard to the efficiency of the technology for DH-plant production was shown as well as advantages and limitations of each technology were described.


Огурец (cucumis sativus l.), dh-растения, культура неопыленных семяпочек in vitro, cucumis sativus l, dh-plant, isolated microspore cultivation in vitro, gynogenesis, parthenogenesis, androgenesis, homozygous lines, (cucumis sativus l.), культура микроспор in vitro


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IDR: 140245781   |   DOI: 10.18619/2072-9146-2019-5-3-14

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