Obtaining the recombinant -l-fucosidase (AlfB)

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In this study the gene α-L-fucosidase (AlfB), amplified by PCR was cloned in the vector plasmid pet23b + sites for EcoR1 - Xho1. Gene expression occurred in the cells of Escherichia coli top 10 under the control of the T7 promoter. The strain-producer recombinant protein α-L-fucosidase was obtained, optimum values of pH and temperature was determined by optimizing the conditions of the enzyme action.

Α-l-фукозидаза, escherichia coli

Короткий адрес: https://sciup.org/14040070

IDR: 14040070

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