Сlonal micropropagation of promising cultivars and forms of half-highbush blueberry using a vitamin-mineral complex

Автор: Makarov S.S., Kuznetsova I.B., Zaushintsena A.V., Kulikova E.I.

Журнал: Лесохозяйственная информация @forestry-information

Рубрика: Охрана и защита лесов

Статья в выпуске: 4, 2021 года.

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The results of studies on growing in vitro plants of half-highbush blueberry of Northblue cultivar and hybrid form 23-1-11 with the use of a vitamin-mineral complex. Regenerated plants are cultivated on nutrient medias WPM, WPM 1/2, and WPM 1/4 supplemented with cytokinin 2-iP at concentrations of 2.0 and 4.0 ml/l. The highest viability (84-90 %) of explants of half-highbush blueberry of Northblue cultivar and hybrid form 23-1-11 are observed when using Chlorine-free Ecosterilizer 1:1, mercuric chloride (0.1 %) and Lizoformin3000 (5 %) as sterilizing agents with a sterilization time of 15 minutes at the stage of introduction into in vitro culture. An increase in the concentration of cytokinin 2-iP from 2.0 to 4.0 ml/l contributed to an increase in the number of microshoots of blueberry plants by 1.1-1.2 times and a decrease in the total length by 1.3-1.6 times at the stage of “actual micropropagation”. The number of half-highbush blueberry micro-shoots increased by 1.0-1.2 times and the total length of shoots by 1.3-2.1 times with the addition of a vitamin-mineral complex. The number and total length of microshoots of blueberry plants of the half-highbush of hybrid form 23-1-11 exceeded than Northblue cultivar by 1.1-1.3 and 1.3-1.4 times respectively.

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In vitro

Короткий адрес: https://sciup.org/143177862

IDR: 143177862   |   DOI: 10.24419/LHI.2304-3083.2021.4.07

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